The gene expressions of 5 cytokines(IL-1, IL-2, IL-6, TNFα and IFNΥ) from human peripheral blood mononuclear cells(PBMC) stimulated with Lentinus edodes lectin(LEL) were investigated by using reverse transcription polymerase chain
reaction(PRT-PCR).
PBMC(1×10E6 cells/ml_ isolated from healthy volunteers were stimulated with LEL(O.D.=3.0) or lipopolysaccharide(LPS, 10μg/ml) for various times(1, 8, 24, 48, 72, 96 and 120 hours). After each of the various stimulated times, total RNA was
isolated by
RNA zol B method and assessed for various cytokines mRNA BY RT-PCR. The mRNA encoding IL-6 and IFNΥwere detected continuously from 1 to 120 hours. The mRNA encoding IL-1 were detected up to 72 hours and the intensity of the band were increased
from 24
to 72 hours. The mRNA encoding IL-2 were detected from 8 to 120 hours and showed delayed relayed reaction compare with other cytokines. But the mRNA encoding TNFα was detected up to 24 hours only. The patterns of TNFαgene expression showed
short
time
response compared with other cytokines.
These results suggest that LEL, as an inducers of cytokines, can elicits a detectable cytokine on mRNA from the PBMC within the fist few hours of stimulation.
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